E. coli Gene Knockout

Introduction

Gene knockout is a powerful tool for studying gene function and has numerous applications in various fields, including basic research, biotechnology, and medicine. E. coli is an ideal organism for gene knockout studies due to its ease of manipulation, fast growth rate, and well-characterized genome. By disrupting specific genes in E. coli, researchers can investigate the role of those genes in various biological processes, including metabolism, protein synthesis, and stress response.

At CD Biosynsis, we offer a range of gene knockout services, including CRISPR-Cas9, homologous recombination, and RNAi, to help researchers unlock the full potential of synthetic biology.

Figure 1: Overview of CRISPR/Cas9 genome editing strategy in Escherichia coli. (König, E., et al. 2018)

Our Services for Gene Knockout in E. coli

At CD Biosynsis, we offer a wide range of gene knockout services for E. coli, including:

CRISPR-Cas9 Gene Knockout

CRISPR-Cas9 is a revolutionary gene editing tool that allows for precise and efficient gene knockout in E. coli. Our experts use this technology to disrupt specific genes of interest, enabling researchers to study the resulting phenotype and its implications for bioproduct development.

Homologous Recombination Gene Knockout

Homologous recombination is another gene knockout method we offer, which involves the use of a selectable marker to replace the target gene with a new gene. This method allows for more precise control over the knockout event and can be used to study the effects of gene knockout on gene expression and protein function.

RNA Interference Gene Knockdown

RNA interference (RNAi) is a gene silencing mechanism that can be used to knockdown specific genes in E. coli. By using RNAi, researchers can investigate the role of specific genes in various biological processes without completely disrupting them.

Our Advantages

Gene knockout in E. coli offers several advantages, including:

• Rapid Knockout: Our gene knockout services allow for rapid knockout of specific genes in E. coli, enabling researchers to quickly study the resulting phenotype and its implications for bioproduct development.
• Easy Manipulation: E. coli is an easy-to-manipulate organism, making it an ideal choice for gene knockout studies. Our experts can easily introduce genetic modifications into E. coli using established transformation protocols.
• Cost-Effective: Gene knockout in E. coli is a cost-effective way to study gene function and develop novel bioproducts. By using gene knockout techniques, researchers can save time and resources compared to traditional gene knockout methods.

Gene knockout in E. coli is a powerful tool for studying gene function and developing novel bioproducts. Our expertise and cost-effective services make us the ideal partner for any gene knockout project in E. coli. Contact us today to learn more about our services and how we can help you.

References

1. König, E., et al. Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli. Bio-protocol, 2018, 8(2), e2688.
2. Jensen, S., et al. Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress. Scientific Reports, 2016, 5, 17874.

Please note that all services are for research use only. Not intended for any clinical use.